Integrins in Cultured Primate Bronchial Epithelial Cells
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چکیده
The effects of 0.5 ppm ozone exposure for 6 h on the synthesis and distribution of b 1 integrins were examined in bronchial epithelial cells cultured at an air–cell interface. Ozone exposure damaged cilia and caused significant cell loss. Immunocytochemical localization and quantification of the b 1 subunit in the remaining attached cells using scanning laser cytometry demonstrated time-dependent changes in b 1 distribution in response to ozone. Although no changes were detected immediately after exposure, b 1 immunoreactivity increased 23 6 5% and 66 6 6% at 6 and 24 h, respectively. The increased immunostaining was localized at the apical surfaces and, to a lesser extent, at cell–cell contacts of cultured cells. Furthermore, integrin redistribution was not due to increased messenger RNA (mRNA) levels and protein synthesis because levels of b 1 mRNA and newly synthesized b 1 protein did not change after ozone exposure. However, immunoprecipitation analysis of b 1 integrins in lysates from equal numbers of cells showed that ozone-exposed cells contained 90 6 15% more total b 1 subunit at 24 h after exposure. In addition, our results demonstrated the presence of the a 5 b 1 integrin complex in bronchial epithelial cells and that the detergent-soluble amount of its associated b 1 subunit increased 60 6 10% in lysates of ozone-exposed cells. In conclusion, ozone altered cellular distribution of b 1 integrins in the remaining attached cells subsequent to cell injury and loss. The changes in b 1 distribution might be due to increased detergent extractibility of b 1 integrins rather than a real increase in the synthesis of b 1 integrins. Jabbour, A. J., L. C. Altman, T. N. Wight, and D. L. Luchtel. 1998. Ozone alters the distribution of b 1 integrins in cultured primate bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 19:357–365.
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تاریخ انتشار 1998